The inflammation process as a part of a complex biological response is characterized by the following phases: alteration, exudation, proliferation, and activation of the immune response. This is the standard behavior of all complex organisms in the course of a disease. Modern treatment of all dangerous diseases includes the usage of anti-inflammation medicines. Despite the natural origin of inflammation, it can seriously injure the patient with the body's depletion of resources and pain syndrome. Both effects are harmful to the regeneration process and the nervous system.
Based on the cell types and molecules involved in the process, inflammation can be classified as either acute or chronic. Different studies of the last decades revealed a group of essential proteins and messengers responsible for developing progressive and slow immune responses. Usually, the treatment of a more acute inflammation process is significantly better manageable because of its rapid and timely diagnosis. At the same time, chronic diseases often demonstrate typical features of general illness and should be diagnosed at an early onset. Such disorders as hay fever, periodontitis, atherosclerosis, rheumatoid arthritis, and even cancer showed the involvement of similar pathways with a range of protein targets engaged in chronic inflammation development.
Life Chemicals has prepared a proprietary Anti-inflammatory Screening Compound Library of over 2,900 drug-like screening compounds with both ligand-based and receptor-based approaches for new anti-inflammatory screening and drug discovery research:
- Anti-inflammatory Focused Compound Library by 2D Fingerprint Similarity Search
- Inflammation Targeted Compound Library by Docking Screening
The compound selection can be customized based on your requirements, cherry-picking is available.
Please, contact us at email@example.com for any additional information and price quotations.
Anti-inflammatory Focused Compound Library by 2D Fingerprint Similarity Search
2D fingerprint similarity implies that a reference set of active compounds (with their activity data reported in the literature) and molecules of interest are represented as a set of small fragments encoded in bit strings (“fingerprints”). Both sets of “fingerprints” are compared to estimate their degree of similarity.
First, a reference set of known molecules modulating inflammation targets was obtained from the ChEMBL database. Next, the 2D fingerprint similarity search against the Life Chemicals HTS Compound Collection was performed with the Tanimoto index ≥ 0.8 (10 analogs max per reference compound), resulting in almost 1,700inflammation-related screening compounds. The library does not contain PAINS, toxic and reactive compounds.
Targets covered with this Library are:
Inflammation Targeted Compound Library by Docking Screening
This screening set was designed based on published data on protein interaction signaling pathways and the protein structural data availability (RCSB protein data bank) along with active protein inhibitors (ChEMBL DB).
Over 1,200 potential anti-inflammatory agents were identified using the docking screening method against the Life Chemicals HTS Compound Collection. The following inflammation-connected molecular targets involved in the activation of other modulators or strengthening defense mechanisms have been considered:
- Phosphatidylinositol-4,5-bisphosphate 3-kinases (PI3K) Alpha and Beta Subunits
- Protein-tyrosine Phosphatase 1B (PTP1B)
- Leukotriene A4 (LTA4)
- Janus Kinase 3 (JAK3)
- Prostaglandin-endoperoxide Synthase 2, Cyclooxygenase-2 (COX-2)
- Sphingosine kinase 1 (SK1)
- Sphingosine-1-phosphate Lyase (SPL1)
- Spleen Tyrosine Kinase (SYK)
- RAR-related orphan receptor gamma (RORγ)
- Leucine-rich repeat kinase 2 (LRRK2)
- Nitric Oxide Synthase Inducible (iNOS)
The presented screening set covers several stages of production and degradation of signaling intermediates (cytokines, phosphatidylinositol, prostaglandin, thromboxane, sphingosine-1-phosphate).
Phosphatidylinositol-4,5-bisphosphate 3-kinases (PI3K) Alpha and Beta Subunits
Phosphatidylinositol-4,5-bisphosphate 3-kinases (also called phosphatidylinositide 3-kinases, PI3K) are a family of enzymes involved in several cellular functions such as cell growth, proliferation, differentiation, motility, survival, and intracellular trafficking. A disorder of each of the pathways may cause cancer development.
These intracellular signal transducer enzymes produce a signaling intermediate by phosphorylating the 3-position hydroxyl group of the inositol ring of phosphatidylinositol. Class IA PI3K is composed of a heterodimer between a p110 catalytic subunit and a p85 regulatory subunit. Many of its functions relate to the ability of PI3-kinases to activate protein kinase B (PKB, aka Akt) as in the PI3K/AKT/mTOR pathway. It is also known that PI3Ks are involved in various immune responses and are produced in different immune cells. A special role of p110δ and p110γ isoforms of PI3K consists in the regulation of multiple aspects of immune defense and inflammation.
The presented Library contains potential small-molecule inhibitors of gamma and delta isoforms of PI3K from class Ia. Based on crystallography data and spatial alignments, the most critical ligand-protein interactions were determined. These data on pharmacophore sites were used in the preparation of an in silico screening model.
Fig. 1. An interaction map of the bound γ-subunit inhibitor CHEMBL394235 (IC50 = 11.8 nM) in the PI3K protein site (PDBID: 4EZK) during validation of the screening model (left). Superposed alignment of known PI3K inhibitor GDC-0326 (in red) and a compound from the Life Chemicals Stock Collection (right, in magenta) in the averaged structure of γ/δ subunits.
Protein-tyrosine Phosphatase 1B (PTP1B)
Tyrosine-protein phosphatase non-receptor type 1, also known as protein-tyrosine phosphatase 1B (PTP1B), is a “primogenitor” member of the protein tyrosine phosphatase (PTP) family of enzymes. The two-stage dephosphorylation mechanism provides engagement of the enzyme in different pathways. Among its substrates, there are several tyrosine kinases (EGFR, c-SRC, JAK2, etc.) and some other tyrosine-phosphorylated proteins (BCAR1, DOK1). Several studies showed that over-expression of PTP1B decreases the level of TNF-α and IL-6 in macrophages. Its essential role in the development of diabetes and obesity was also proven. However, there is no evident data concerning the mechanism of such regulations. PTP1B plays a central role in producing pro-inflammatory cytokines in microglia through modulation of Src activity.
Due to a specific active site, selectivity is one of the major issues in the development of PTP1B inhibitors as pharmaceutical drugs. Two binding modes were developed based on a list of crystal structures and interaction maps to fully cover the prospective chemical space. Both screening models were validated with a set of potent reference compounds.
Fig. 2. Comparative demonstration of the docked Life Chemicals hit compound (left) and the reference compound from ChEMBL (right).
Leukotriene A4 (LTA4)
Leukotriene A4 (LTA4) hydrolase is a zinc-containing bifunctional enzyme that converts leukotriene A4 to leukotriene B4 and acts as an aminopeptidase (epoxide LTA4 to the diol leukotriene B4 (LTB4). The LTB4 plays a significant role in the further development of many inflammatory disease conditions. As shown during the last 10-15 years, all therapeutic agents, which selectively inhibit LTA4 hydrolase and inhibit the formation of LTB4, could be potentially helpful for the inflammation process suppression. The first inhibitors of LTA4 were based on the structure of a natural substrate. The next generation of peptide and non-peptide analogs was designed to mimic the substrate. They contain potential zinc-chelating moieties, including thiols, hydroxamates, and norstatines.
Considering the literature data and activity of experimentally approved drugs, we applied both structure-based and ligand-based approaches to generate docking models. Metal-binding sites of the ligands, hydrophobic regions, and H-bonds that they are forming with a protein molecule were used to produce accurate and exhaustive docking.
Fig. 3. Two hits from the Life chemicals Stock, bound to the Zn atom from the opposite direction in the active site of LTA4 (PDBID:3FH7) (upper row), and two similar docked inhibitors from the ChEMBL database (CHEMBL515470, IC50 = 29 nM and CHEMBL481860 IC50 = 46 nM) in the same positions (bottom row).
Janus Kinase 3 (JAK3)
The Janus kinases (JAK 1,2,3) are a family of intracellular tyrosine kinases that play an essential role in signaling cytokines implicated in the inflammatory process. All members of the family initiate binding of specific receptors with a broad array of cytokines. All of them are involved in the inflammation process activation. For example, they have a crucial function in Hemophagocytic lymphohistiocytosis (HLH).
While inhibition of JAK1 and JAK2 can cause drug-related adverse events (e.g., overt immunosuppression, anemia), Janus Kinase 3 (JAK3), a hematopoietic cell-restricted tyrosine kinase, represents an attractive target for immunosuppression due to its limited tissue distribution and specific role in lymphoid homeostasis. Such JAK3 inhibitor as CP-690 550 has shown efficacy as an immunosuppressant.
Life Chemicals has prepared a library of potentially selective JAK3 inhibitors employing in silico screening and exhaustive docking procedures. Ligand efficiency was estimated by docking of the reference compounds.
Fig. 4. Docked reference compounds CHEMBL577944 (IC50 = 500 nM) and CHEMBL2062809 (IC50 = 72 nM) (left) and a hit from the Life Chemicals HTS Compound Collection (right).
Prostaglandin-endoperoxide Synthase 2, Cyclooxygenase-2 (COX-2)
Prostaglandin-endoperoxide synthase 2 (cyclooxygenase-2 or COX-2) is an enzyme that plays a crucial role in inflammatory processes by conversion of arachidonic acid to prostaglandin H2, which is an important precursor of prostaglandins (such as prostacyclin) and thromboxane A2. Hence, inhibition of COX can provide relief from inflammation and pain symptoms. This enzyme contains a short N-terminal epidermal growth factor (EGF) domain, an α-helical membrane-binding moiety, and a C-terminal catalytic domain. Human PTGS2 (COX-2) functions as a conformational heterodimer, having a catalytic monomer and an allosteric monomer (E-cat) and E-allo. Heme binds only to the catalytic part’s peroxidase site, while substrates and known inhibitors bind to the COX site of the catalytic part. E-cat is regulated by E-allo in a way dependent on a ligand to be bound to E-allo.
Several studies showed that inflammation proteins (like Prostaglandin endoperoxide H synthases) are also targets of COX-2. Inhibition of COX-2 that is selectively induced by pro-inflammatory cytokines at the inflammation site increases the anti-inflammatory properties effect. One of the benefits of targeting COX-2 is its usual specific localization in inflamed tissue, so it causes much less gastric irritation associated with COX-2 inhibitors. It was also reported that selective inhibition of PTGS2 (COX-2) shows fewer side effects than inhibition of the COX-1 enzyme.
Fig. 5. Several docked reference compounds aligned in the active site of COX-2 protein (PDBID:5KIT) (left) and the same view for docked hits from the Life Chemicals Stock Collection (right).
Sphingosine Kinase 1 (SK1)
Sphingosine kinase 1 phosphorylates sphingosine to sphingosine-1-phosphate (S1P). Sphingosine kinase 1 is usually a cytosolic protein but is recruited to membranes rich in phosphatidate (PA), a product of Phospholipase D (PLD). Its substrate, Sphingosine-1-phosphate (S1P), is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates the cell’s proliferation and survival, and extracellularly, it is a ligand for Sphingosine-1-phosphate receptor 1, providing an inflammation signaling.
Sphingosine kinases (SKs) and their lipid product S1P play essential roles in inflammatory signaling processes, as well as disease development and progression. Systemic lupus erythematosus, arthritis, ulcerative colitis, and Crohn’s disease are among disorders caused by SK/S1P cascade activation. SKs can be activated by numerous growth factors and cytokines. Both isoforms of SK (SK1 and SK2) can phosphorylate sphingosine to form S1P, however, their substrate specificity is different.
This Library includes compounds selected by in silico screening and contains novel chemical structures with well-predicted affinity to the active site.
Fig. 6. Similar location and orientation of a reference compound CHEMBL2409849 (IC50 = 4 nM) (left) and one of the hits from the Life Chemicals Collection (right) in the binding site of Sphingosine Kinase 1 protein (PDBID: 4l02)
Sphingosine-1-phosphate Lyase (SPL1)
Sphingosine-1-phosphate lyase (SPL), a membrane-bound enzyme of sphingolipid metabolism, catalyzes irreversible degradation of sphingoid base phosphates. Its primary substrate, sphingosine-1- phosphate, a polar sphingolipid metabolite, acts both extracellularly by binding G protein-coupled receptors of the lysophospholipid receptor family inside the cell as a second messenger. SPL is expressed in many mammalian tissues, and S1P tissue level is usually low and kept under control through equilibrium between its synthesis mainly governed by sphingosine kinase-1 (SK1) and its degradation by sphingosine 1-phosphate lyase (SPL). Inhibitors of SPL may become potent therapeutic agents for a variety of diseases in which S1P is involved.
Based on the crystal structure of the ligand and its interaction map, Life Chemicals has designed a library comprising compounds with excellent 3D pharmacophore matching.
Fig. 7. Reference compounds docked into the active site (left) and one of the hits aligned to the co-crystalized ligand of SPL (PDB ID: 4Q6R)
Spleen Tyrosine Kinase (SYK)
Spleen tyrosine kinase (SYK) is a member of the SYK family of tyrosine kinases. Tyrosine kinases are enzymes that catalyze the ligand’s crystal structure phosphorylation of tyrosine residues on protein substrates, involved in signaling pathways that drive various cellular responses, including proliferation, differentiation, migration, and survival.
The structure of these non-receptor cytoplasmic tyrosine kinases shares a characteristic dual SH2 domain separated by a linker domain. SH2 domains typically bind to phosphorylated tyrosine residues within a motif of a target protein. This interaction initiates a cascade of events, inducing several cellular responses. SYK couples immune cell receptors to intracellular signaling pathways that regulate cellular responses on the activated inflammation process. Due to its overexpression in hematopoietic cells, SYK is considered a pro-survival factor for several hematological and non-hematological cancers. SYK kinases are promising therapeutic targets for disorders such as arthritis, allergic conditions, idiopathic thrombocytopenic purpura, systemic lupus erythematosus, B-cell lymphoma.
The target’s structural and functional features open up an opportunity to use small-molecule inhibitors of SYK for the treatment of both immune disorders and cancer.
Fig. 8. An interaction map of the known SYK inhibitor and one of the screened compounds in the active site of the kinase (PDBID: 4XG9)
RAR-related orphan receptor gamma (RORγ)
RAR-related orphan receptor gamma (RORγ or RORC) is a member of a nuclear receptor family specifically expressed in T cell compartments. Two isoforms, RORγt and RORγ, are encoded by a single gene called Rorg. The difference between these isoforms is in the length of the N-terminus and the more restricted distribution of the second one. One of the most interesting functions of RORγ suggests a conversion of CD4⁺ immune cells into pro-inflammatory Th17 cells. Interleukin-17 (IL-17) and T helper 17 (TH17) cells play a crucial role in tissue inflammation. So, the prevention of cytokines' overproduction can regulate immune responses.
It was shown that RORγ takes part in many autoimmune disorders, like psoriasis, psoriatic arthritis, ankylosing spondylitis, inflammatory bowel disease, and multiple sclerosis. Despite several novel medicines developed and tested during the last decade, a considerable molecular weight of antibodies makes them unsuitable for usage as topical medicines because they cannot diffuse across the skin barrier. Another way to reduce symptoms involves modulation of RORγ transcriptional activity by interfering with RORγ-DNA binding to decrease cytokine transcription level.
Therefore, local inhibition of RORγ/RORγt with small molecular weight inhibitors represents a unique approach to selective inhibition of aberrant IL-17 cytokine production.
Fig. 9. The docking model’s evaluation by alignment and scoring of reference compounds (left) and demonstration of pharmacophore matching to the hit compound from the Life Chemicals Stock (right). The template taken from the crystal structure is colored with cyan.
Leucine-rich repeat kinase 2 (LRRK2)
Leucine-rich repeat kinase 2 (LRRK2), also known as dardarin, belongs to the Ras of a complex protein family, which is characterized by the presence of a Ras-like G-domain (Roc), a C-terminal of Roc domain (COR), and a kinase domain. Its active form is a dimer, whose functions are similar to serine/threonine-specific kinase. Expression of LRRK2 mutants has been found to be the most frequent cause of Parkinson’s disease with a shortening and simplification of the dendritic tree.
LRRK2 takes place in some pathways involved in neuronal damage, including the microtubule network, actin cytoskeleton reorganization, autophagy, mitochondria, vesicular trafficking, and protein quality control. It is also a novel regulator of the Nuclear factor of activated T-cells (NFAT) that modulates the severity of inflammatory bowel disease. The mechanism of its action is not thoroughly investigated, but the knockout or pharmacological inhibition of LRRK2 results in defects in synaptic vesicle endocytosis, altered synaptic morphology, and neurotransmission impairments. It is suggested that because of the kinase nature of LRRK2, it might modulate the phenotype of microglia through hyperpolymerization and hyperphosphorylation of cytoskeleton and vesicle components, thus pushing these cells toward a pro-inflammatory state.
Based on a reference set of biologically active compounds (ChEMBL DB, KI, and IC50 values) clustered by structure, five diverse scaffolds were identified. A conformer search calculation and cross-alignment were carried out to identify a possible active conformation for each cluster’s core structures. Five different QSAR models were built and validated for subsequent prediction of structurally-diverse screening compounds with potential LRRK2 inhibitory activity from the Life Chemicals HTS Compound Collection.
Fig.10. An example of conformational search pull with an electrostatic surface (left) and randomly chosen hits from the Life Chemicals HTS Compound Collection.
Nitric Oxide Synthase Inducible (iNOS)
Nitric oxide synthases (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. It helps modulate vascular tone, insulin secretion, airway tone, and peristalsis and is involved in angiogenesis and neural development. It may function as a retrograde neurotransmitter, and it is an important cellular signaling molecule.
There are three known isoforms in mammals, two are constitutive (cNOS), and the third is inducible (iNOS). iNOS has been described as calcium-insensitive due to its tight non-covalent interaction with calmodulin (CaM) and Ca2+. The expression of the inducible nitric oxide synthase (iNOS) is one of the markers of an inflammatory process, contributing to local tissue destruction during chronic inflammation.
The Life Chemicals HTS Compound Collection was screened, using the model, which contains obligatory interactions with two key-role residues in the active site and/or metal-binding ability.
Fig. 11. iNOSbinding site surface with docking constraints (spheres and asterisks) covers both re-docked inhibitors from crystal structures (wireframe) and one of the compounds from the Life Chemicals HTS Compound Collection (balls and sticks).
- Carpenter CL, Duckworth BC, Auger KR, Cohen B, Schaffhausen BS, Cantley LC (Nov 1990). "Purification and characterization of phosphoinositide 3-kinase from rat liver". The Journal of Biological Chemistry. 265 (32): 19704–11. PMID 2174051.
- P.T. Hawkins, L.R. Stephens (Jun 2015). "PI3K signaling in inflammation." Molecular and Cell Biology of Lipids. Volume 1851,Issue 6,Pages 882–897
- Rommel C1, Camps M, Ji H. PI3K delta and PI3K gamma: partners in crime in inflammation in rheumatoid arthritis and beyond? Nat Rev Immunol. 2007 Mar;7(3):191-201. Epub 2007 Feb 9.
- P G Través, V Pardo, M Pimentel-Santillana1, Á González-Rodríguez, M Mojena, D Rico, Y Montenegro1, C Calés1, P Martín-Sanz1, A M Valverde1 and L Boscá1. Pivotal role of protein tyrosine phosphatase 1B (PTP1B) in the macrophage response to pro-inflammatory and anti-inflammatory challenge. Cell Death and Disease (2014) 5, 13 March 2014.
- Zabolotny JM, Kim YB, Welsh LA, Kershaw EE, Neel BG, Kahn BB. "Protein-tyrosine phosphatase 1B expression is induced by inflammation in vivo." J Biol Chem. 2008 May 23;283(21):14230-41. Epub 2008 Feb 14.
- Gyun Jee Song, Myungsu Jung, Jong-Heon Kim, Hana Park, Md Habibur Rahman, Sheng Zhang, Zhong-Yin Zhang, Dong Ho Park, Hyun Kook, In-Kyu Lee and Kyoungho SukEmail. "A novel role for protein tyrosine phos- phatase 1B as a positive regulator of neuroinflammation.", Journal of Neuroinflammation201613:86
- Rao NL1, Dunford PJ, Xue X, Jiang X, Lundeen KA, Coles F, Riley JP, Williams KN, Grice CA, Edwards JP, Karlsson L, Fourie AM.J "Anti-inflammatory activity of a potent, selective leukotriene A4 hydrolase inhibitor in comparison with the 5-lipoxygenase inhibitor zileuton." Pharmacol Exp Ther. 2007 Jun;321(3):1154-60. Epub 2007 Mar 19.
- Thomas D. Penning "Inhibitors of Leukotriene A4 (LTA4) Hydrolase as Potential Anti-Inflammatory Agents." Curr Pharm Des. 2001 Feb;7(3):163-79.
- Stsiapanava A, Olsson U, Wan M, Kleinschmidt T, Rutishauser D, Zubarev RA, Samuelsson B, Rinaldo-Matthis A, Haeggström JZ."Binding of Pro-Gly-Pro at the active site of leukotriene A4 hydrolase/aminopeptidase and devel- opment of an epoxide hydrolase selective inhibitor." Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4227-32.
- Lalitha Vijayakrishnan, R. Venkataramanan, Palak Gulati. "Treating inflammation with the Janus Kinase inhibitor CP-690,550". Trends in Pharmacological Sciences, Volume 32, Issue 1, p25–34, January 2011
- Das R, Guan P, Sprague L, Verbist K, Tedrick P, An QA, Cheng C, Kurachi M, Levine R, Wherry EJ, Canna SW, Behrens EM, Nichols KE. "Janus kinase inhibition lessens inflammation and ameliorates disease in murine models of hemophagocytic lymphohistiocytosis." Blood. 2016 Mar 31;127(13):1666-75.
- Seibert K, Masferrer JL. "Role of inducible cyclooxygenase (COX-2) in inflammation." Receptor. 1994 Spring;4(1):17-23.
- Takashi Kuwano, Shintaro Nakao, Hidetaka Yamamoto, Masazumi Tsuneyoshi, Tomoya Yamamoto, Michihiko Kuwano and Mayumi Ono. "Cyclooxygenase 2 is a key enzyme for inflammatory cytokine-induced angiogenesis." FASEB J. 2004 Feb;18(2):300-10.
- Alhouayek M, Muccioli GG2. "COX-2-derived endocannabinoid metabolites as novel inflammatory mediators." Trends Pharmacol Sci. 2014 Jun;35(6):284-92. Epub 2014 Mar 29.
- Ashley J Snider. "Sphingosine kinase and sphingosine-1-phosphate: regulators in autoimmune and inflammatory disease." Int J Clin Rheumtol. 2013 Aug 1; 8(4): 10.2217/ijr.13.40.
- Geng T, Sutter A, Harland MD. "SphK1 mediates hepatic inflammation in a mouse model of NASH induced by high saturated fat feeding and initiates proinflammatory signaling in hepatocytes." J Lipid Res. 2015 Dec;56(12):2359-71.Epub 2015 Oct 19.
- Fischer I, AlliedC, Martinier N. "Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions." PLoS One. 2011;6(8):e23905. Epub 2011 Aug 24.
- Ceccom J, Loukh N, Lauwers-Cances V. "Reduced sphingosine kinase-1 and enhanced sphingosine 1-phosphate lyase expression demonstrate deregulated sphingosine 1-phosphate signaling in Alzheimer's disease." Acta Neuropa- thol Commun. 2014 Jan 27;2:12.
- Bourquin F, Riezman H. "Structure and function of sphingosine-1-phosphate lyase, a key enzyme of sphingolipid metabolism." Structure. 2010 Aug 11;18(8):1054-65.
- Montserrat Serra and Julie D. Saba. "Sphingosine 1-phosphate lyase, a key regulator of sphingosine 1-phosphate signaling and function." Adv Enzyme Regul. 2010; 50(1): 349–362.
- Riccaboni M, Bianchi I, Petrillo P. "Spleen tyrosine kinases: biology, therapeutic targets, and drugs." Drug Discov Today. 2010 Jul;15(13-14):517-30. Epub 2010 May 27.
- Geahlen RL. "Getting SYK: spleen tyrosine kinase as a therapeutic target." Trends Pharmacol Sci. 2014 Aug;35(8):414-22. Epub 2014 Jun 26.
- Kuan-Hsing Chen, Hsiang-Hao Hsu, Huang-Yu Yang. "Inhibition of spleen tyrosine kinase (SYK) suppresses renal fibrosis through anti-inflammatory effects and downregulation of the MAPK-p38 pathway." The International JoJournalf Biochemistry & Cell Biology. Volume 74, May 2016, Pages 135–144
- Susan H. Smith, Carlos E. Peredo, Yukimasa Takeda, Thi Bui. "Development of a Topical Treatment for Psoriasis Targeting RORγ: From Bench to Skin." PLoS One. 2016 Feb 12;11(2):e0147979.
- Huang Z, Xie H, Wang R, Sun Z. "Retinoid-related orphan receptor gamma t is a potential therapeutic target for controlling inflammatory autoimmunity." Expert Opin Ther Targets. 2007 Jun;11(6):737-43.
- Isabella Russo, Luigi Bubacco. "LRRK2 and neuroinflammation: partners in crime in Parkinson’s disease?" J Neuroinflammation. 2014; 11: 52.
- Bernd K. Gilsbach and Arjan Kortholt. "Structural biology of the LRRK2 GTPase and kinase domains: implications for regulation." Front Mol Neurosci. 2014; 7: 32.
- Kathrin Brockmann, Anja Apel, Claudia Schulte1. "Inflammatory profile in LRRK2-associated prodromal and clinical PD." Brockmann et al. Journal of Neuroinflammation (2016) 13:122
- Curr Mol Med. 2004 Nov;4(7):763-75. "The role of iNOS in chronic inflammatory processes in vivo: is it damage- promoting, protective, or active at all?" Suschek CV1, Schnorr O, Kolb-Bachofen V.
- Mediators of Inflammation. Volume 2015 (2015), Article ID 138461, 9 pages. "iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants." Puebla Cassini-Vieira, Fernanda Assis Araújo